Web-based key for the identification of Rotylenchus spp.

Please fill in your matrix codes following the matrix code of Castillo and Vovlas (2005):


A) Lip annulation:
– 1: absent or smooth labial region
– 2: labial region with 2-3 annuli
– 3: labial region with four annuli
– 4: labial region with five annuli
– 5: labial region with six annuli
– 6: labial region with 7-8 annuli
– 7: labial region with 9-10 annuli
B) Labial region shape:
– 1: labial region hemispherical
– 2: labial region rounded
– 3: labial region conoid
– 4: labial region truncate
C) Lateral field areolation:
– 1: areolated only in pharyngeal region
– 2: areolated in pharyngeal region and irregularly areolated at mid-body
– 3: areolated in pharyngeal region and incompletely areolated at mid-body
– 4: areolated in pharyngeal region and near phasmids
– 5: areolated along whole length of body except at tail region
– 6: areolated along whole length of body including the tail region
– 7: incompletely areolated along whole body
D) Body longitudinal striations:
– 1: punctated along body annuli
– 2: longitudinally striated in pharyngeal region
– 3: longitudinally striated over whole body
– 4: without body striations
E) Stylet length:
– 1: stylet shorter than 30 micrometer
– 2: stylet between 30 and 35.9 micrometer
– 3: stylet between 36 and 40.9 micrometer
– 4: stylet longer than 41 micrometer
F) Dorsal pharyngeal gland outlet to stylet base:
– 1: DGO less than 2 micrometer
– 2: DGO between 2 and 6.9 micrometer
– 3: DGO between 7 and 12 micrometer
– 4: DGO >12 micrometer
G) Dorsal pharyngeal gland overlapping:
– 1: pharyngeal gland overlapping less than 5 micrometer
– 2: pharyngeal gland overlapping by 6-20.9 micrometer
– 3: pharyngeal gland overlapping by 21-30.9 micrometer
– 4: pharyngeal gland overlapping by 31-40.9 micrometer
– 5: pharyngeal gland overlapping by > 41 micrometer
H) Tail shape:
– 1: hemispherical
– 2: rounded
– 3: conoid
– 4: pointed
– 5: with ventral projection
I) Ratio V:
– 1: ratio V < 50%
– 2: ratio V of 50-70%
– 3: ratio V > 70%
J) Presence of males:
– 1: males present
– 2: males absent
K) Phasmid position:
– 1: well anterior to level of anus (> five annuli anterior to anus)
– 2: at level of anus (from five annuli anterior to five annuli posterior to anus)
– 3: well posterior to level of anus (> five annuli posterior to anus)





Expected Bray-Curtis similarity: %





Note: feature D in the matrix code for R. goodeyi has been changed from D4–>D2 due to the mistake of Castillo and Vovlas in coding

Contact: tien.quelampb@gmail.com

Please cite:

1. Nguyen, H.T., Trinh Q.P., Couvreur, M., Singh, P.R., Decraemer, W. and Bert, W. 2019. Description of Rotylenchus rhomboides n. sp. and a Belgian population of Rotylenchus buxophilus (Tylenchomorpha: Hoplolaimidae). Journal of nematology. 51: e2019-23.

2. Nguyen, H.T., Trinh Q.P., Couvreur, M., Singh, P.R., Decraemer, W. and Bert, W. 2019. Molecular and morphological characterisation of a new root-lesion nematode, Pratylenchus horti n. sp. (Tylenchomorpha: Pratylenchidae) from Ghent University Botanical Garden. Nematology. 21, 739-752.